Journal: Biochemical and biophysical research communications
Article Title: Sialic Acid-Dependent Attachment of Mucins from Three Mouse Strains to Entamoeba histolytica
doi: 10.1016/j.bbrc.2013.05.085
Figure Lengend Snippet: Entamoeba histolytica membrane binding inhibition assay using a neo-glycoprotein (A) Schema of the inhibition assay. (B) B6 mucins treated with sialidase [B6 (+SA)] or heat-inactivated sialidase [B6 (−SA)] were incubated with E. histolytica membranes and loaded onto a (GalNAc)12-27-HSA-coated microtiter plate. Six independent experiments were conducted for each condition. The results are shown as a scatter plot with standard deviations. The results obtained with (C) CBA mucins and (D) BALB mucins. Asterisks indicate significant differences between sialidase- and inactivated-sialidase-treated BALB mucins, as determined in a Mann-Whitney’s U-test (*: p<0.05, **: p<0.01).
Article Snippet: The mucin-containing fractions confirmed by PAS staining (Pierce glycoprotein staining kit, Thermo) were concentrated 20-fold using an Amicon Ultracel-100 K (Millipore) and directly used in binding inhibition assays or exchanged with 50 mM ammonium bicarbonate (ABC) for glycoblotting.
Techniques: Membrane, Binding Assay, Inhibition, Incubation